How to Get Best Images with Various Types of Immersion Media and Clearing Agents
In recent years, light sheet fluorescence microscopy (LSFM) with its unique illumination principle has become a standard method to image large optically cleared specimens in toto, and with subcellular resolution. ZEISS Lightsheet 7 can be equipped with dedicated optics, sample chambers and sample holders to accurately adjust to the refractive index of all common clearing methods and immersion media to allow imaging of large samples, even whole mouse brains. In this seminar, we will explain the fundamental principles of sample clearing with a range of use application examples and decision making protocols for determining an optimal clear method for your sample. Understanding the optical principles and choosing appropriate components and imaging settings will help to improve the image quality substantially.
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