You are invited to a FIB Webinar Presentation: 3D tomography of biological samples in the Xe plasma FIB-SEM presented by Dr Crystal Cooper from the Central Analytical Facility, Queensland University of Technology.
Three dimensional (3D) tomography of biological specimens has always been challenging but it is essential to understand morphology, biology, and the associated interactions occurring in various tissues and systems.
Over the last decade there has been an influx of focussed ion beam scanning electron microscopy (FIB-SEM) datasets, mostly generated with a Gallium FIB-SEM. The introduction of the Xenon plasma FIB-SEM allows for the investigation of larger samples (> 20 µm3) as it mills much faster than Gallium.
Xenon is more chemically inert and less conductive than Gallium resulting in the introduction of fewer artefacts. However, using a Xenon FIB-SEM comes with different challenges. Here we look at different biological material investigated using the Xenon FIB-SEM focusing on sample preparation and data acquisition.